Volume 7, Issue 2 (5-2019)                   Iran J Health Sci 2019, 7(2): 39-48 | Back to browse issues page


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School of Public Health, Iran University of Medical Sciences, Tehran, Iran , jonidi.a@iums.ac.ir
Abstract:   (431 Views)
Background and purpose: Bioaerosols enter into human body through various ways (inhalation, ingestion or skin absorption). The aim of this study was to investigate the type and density of the predominant bacteria in two seasons - cold and warm - in the kitchen air of restaurants.
Materials and Methods: This descriptive cross-sectional study was conducted on the air of restaurants' kitchen in Babol. Sampling was performed by using a personal sampling pump with impinger tank using a flow rate of 4 l/min during 50 minutes. Counting the colonies in the air was determined in terms of CFU/m3 and the type of grown bacteria was identified using different tests, such as Gram staining and biochemical methods. Temperature and humidity were recorded at the time of sampling, too. Totally, 120 samples of bacteria were taken from the indoor air of kitchens with and without air conditioning.
Results: The results showed that the highest and lowest densities of bacteria in cold season were 15 CFU/m3 and 63.7 CFU/m3, and in warm season, they were 19.6 CFU/m3 and 80 CFU/m3, respectively. The predominant bacteria were Gram-positive bacteria in the air of kitchen but Bacillus and Micrococcus were the most frequent.
Conclusion: The results showed that due to humidity and the temperature (warmer), bacterial density was higher in summer than winter. The concentration of bacteria in the kitchen and restaurant was also less than WHO recommendation and guide values ACGIH (up to 500 CFU/m3), respectively. So, air quality was found to be good and acceptable in terms of the restaurant kitchen.
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References
1. Chao HJ, Schwartz J, Milton DK, Burge HA. The work environment and workers' health in four large office buildings. Environmental Health Perspectives. 2003;111(9):1242-8. [DOI:10.1289/ehp.5697] [PMID] [PMCID]
2. Sadeghi Hasanvand Z, Sekhavatjo MS. Assessment the bio-aerosols type and concentration in various wards of Valiasr Hospital, Khorramshahr during 2011. Iranian journal of health and environment. 2013;6(2):201-10.
3. Hussin NHM, Sann LM, Shamsudin MN, Hashim Z. Characterization of bacteria and fungi bioaerosol in the indoor air of selected primary schools in Malaysia. Indoor and Built Environment. 2011;20(6):607-17. [DOI:10.1177/1420326X11414318]
4. Nourmoradi H, Nikaeen M, Amin MM, Hatamzadeh M. An Investigation on Bio-aerosol Concentrations in the Different Wards of Hospitals of Isfahan University of Medical Sciences. Journal of Isfahan Medical School. 2011;29(149).
5. Sekhavatjou MS, Zekavati R, Peymani Froshani M. Investigation on particle matter concentration and bacterial bioaerosols in indoor air of prisons. Iranian Journal of Health and Environment. 2013;6(3):295-306.
6. Albrecht A, Fischer G, Brunnemann-Stubbe G, Jäckel U, Kämpfer P. Recommendations for study design and sampling strategies for airborne microorganisms, MVOC and odours in the surrounding of composting facilities. International journal of hygiene and environmental health. 2008;211(1-2):121-31. [DOI:10.1016/j.ijheh.2007.05.004] [PMID]
7. Osaro EF, Ufuoma IO, Dorcas AO. Hospital indoor airborne microflora in private and government owned hospitals in Benin City, Nigeria. World J Med Sci. 2008;3(1):19-23.
8. Salvato JA, Nemerow NL, Agardy FJ. Environmental engineering: John Wiley & Sons; 2003.
9. World Health Organization, Indoor Air Quality: Biological Contaminants. Copenhagen: WHO Regional Publications, European Series No31. 1990.
10. Soleimani Z, Parhizgari N, Rad HD, Akhoond MR, Kermani M, Marzouni MB, et al. Normal and dusty days comparison of culturable indoor airborne bacteria in Ahvaz, Iran. Aerobiologia. 2015;31(2):127-41. [DOI:10.1007/s10453-014-9352-4]
11. Franck U, Herbarth O, Röder S, Schlink U, Borte M, Diez U, et al. Respiratory effects of indoor particles in young children are size dependent. Science of the Total Environment. 2011;409(9):1621-31. [DOI:10.1016/j.scitotenv.2011.01.001] [PMID]
12. Haliki-Uztan A, Ateş M, Abaci Ö, Gülbahar O, Erdem N, Çiftçi Ö, et al. Determination of potential allergenic fungal flora and its clinical reflection in suburban elementary schools in Izmir. Environmental monitoring and assessment. 2010;168(1-4):691-702. [DOI:10.1007/s10661-009-1144-x] [PMID]
13. Mandal J, Brandl H. Bioaerosols in indoor environment-a review with special reference to residential and occupational locations. The Open Environmental & Biological Monitoring Journal. 2011;4(1). [DOI:10.2174/1875040001104010083]
14. Balasubramanian R, Nainar P, Rajasekar A. Airborne bacteria, fungi, and endotoxin levels in residential microenvironments: a case study. Aerobiologia. 2012;28(3):375-90. [DOI:10.1007/s10453-011-9242-y]
15. Faridi S, Hassanvand MS, Naddafi K, Yunesian M, Nabizadeh R, Sowlat MH, et al. Indoor/outdoor relationships of bioaerosol concentrations in a retirement home and a school dormitory. Environmental Science and Pollution Research. 2015;22(11):8190-200. [DOI:10.1007/s11356-014-3944-y] [PMID]
16. Chan P, Yu P, Cheng Y, Chan C, Wong P. Comprehensive characterization of indoor airborne bacterial profile. Journal of Environmental Sciences. 2009;21(8):1148-52. [DOI:10.1016/S1001-0742(08)62395-5]
17. Anwar T. Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh: BRAC Univeristy; 2018.
18. Kim K-Y, Kim H-T, Kim D, Nakajima J, Higuchi T. Distribution characteristics of airborne bacteria and fungi in the feedstuff-manufacturing factories. Journal of hazardous materials. 2009;169(1-3):1054-60. [DOI:10.1016/j.jhazmat.2009.04.059] [PMID]
19. Fang Z, Ouyang Z, Zheng H, Wang X, Hu L. Culturable airborne bacteria in outdoor environments in Beijing, China. Microbial Ecology. 2007;54(3):487-96. [DOI:10.1007/s00248-007-9216-3] [PMID]
20. Augustowska M, Dutkiewicz J. Variability of airborne microflora in a hospital ward within a period of one year. Annals of Agricultural and Environmental Medicine. 2006;13(1):99-106.
21. National Environmental Agency, Institute of Environmental Epidemiology, Ministry of the Environment. Guidelines for good indoor air quality in office premisesFirst edition, pp 44.
22. Mentese S, Rad AY, Arısoy M, Güllü G. Multiple comparisons of organic, microbial, and fine particulate pollutants in typical indoor environments: diurnal and seasonal variations. Journal of the Air & Waste Management Association. 2012;62(12):1380-93. [DOI:10.1080/10962247.2012.714717]
23. Tsai F, Macher J. Concentrations of airborne culturable bacteria in 100 large US office buildings from the BASE study. Indoor air. 2005;15(9):71-81. [DOI:10.1111/j.1600-0668.2005.00346.x] [PMID]
24. Dehdashti A, Sahranavard N, Rostami R, Barkhordari A, Banayi Z. Survey of bioaerosols type and concentration in the ambient air of hospitals in Damghan, Iran. Occupational medicine quarterly Journal. 2013;4(3):41-51.
25. Mushtaq S, Haider MS, Mukhtar I, Khokhar I. Species of deuteromycetes (Fungi imperfect) present at residential houses in Railway colony Lahore, Pakistan. Pak J Phytopathol. 2011;23(2):118-24.
26. Mentese S, Arisoy M, Rad AY, Güllü G. Bacteria and fungi levels in various indoor and outdoor environments in Ankara, Turkey. Clean-Soil, Air, Water. 2009;37(6):487-93. [DOI:10.1002/clen.200800220]
27. Gόrny RL, Dutkiewicz J. Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. Ann Agric Environ Med. 2002;9:17-23.
28. Jo W-K, Seo Y-J. Indoor and outdoor bioaerosol levels at recreation facilities, elementary schools, and homes. Chemosphere. 2005;61(11):1570-9. [DOI:10.1016/j.chemosphere.2005.04.103] [PMID]
29. Aydogdu H, Asan A, Otkun MT. Indoor and outdoor airborne bacteria in child day-care centers in Edirne City (Turkey), seasonal distribution and influence of meteorological factors. Environmental monitoring and assessment. 2010;164(1-4):53-66. [DOI:10.1007/s10661-009-0874-0] [PMID]
30. Awad AHA. Airborne dust, bacteria, actinomycetes and fungi at a flourmill. Aerobiologia. 2007;23(1):59. [DOI:10.1007/s10453-007-9049-z]
31. Yassin M, Almouqatea S. Assessment of airborne bacteria and fungi in an indoor and outdoor environment. International Journal of Environmental Science & Technology. 2010;7(3):535-44. [DOI:10.1007/BF03326162]