en آموزش بهداشت Health Education پژوهشي Original Article <p dir="ltr" style="text-align: justify;">as Pseudomonas, Acinetobacter, and Staphylococcus that appropriate disinfection can reduce&nbsp;these pathogens. The aim of this study was to evaluate the effect of different disinfectants on&nbsp;Gram-negative bacteria isolated from the surface of accidents and burn hospital in Yazd.<br> <strong>Materials and Methods:</strong> In this study, 240 samples were randomly collected from different parts of&nbsp;accidents and burn hospital before and after disinfection. The samples were cultured on blood agar&nbsp;and Eusion-Metilen-Blue agar media in the Microbiology Laboratory of Medicine School of Shahid<br> Sadoughi University in Yazd and Colony counting were determined. Identification was done by&nbsp;biochemical tests after incubation at 37&deg; C for 48 hours. The studied disinfectants were Deconex&nbsp;50AF, Descoscid, Epimax SC, and Silvosept. At last, data were analyzed with using paired t-test.<br> <strong>Results</strong>: The Gram-negative bacteria were isolated from burn unit before disinfection included&nbsp;Pseudomonas aeruginosa, Escherichia coli, Proteus spp., Klebsiella spp., Acinetobacter spp., and&nbsp;Enterobacter spp. According to the results, all disinfectants reduced the pollution before and&nbsp;after disinfection; nevertheless, this reduction at the time of using Epimax SC and Silvosept only&nbsp;showed a significant difference for P. aeruginosa (P = 0.001 and 0.003) and for E. coli&nbsp;(P = 0.020 and 0.005), respectively.<br> <strong>Conclusion</strong>: All disinfectants were effective on Gram-negative bacteria isolated from surfaces&nbsp;and had shown a significant difference only between P. aeruginosa and E. coli number before&nbsp;and after disinfection by Epimax SC and Silvosept. The most effective disinfectant on P.&nbsp;aeruginosa and E. coli was Epimax SC and Silvosept, respectively.</p> Burn Unit, Disinfectant, Gram-negative Bacteria 45 52 http://jhs.mazums.ac.ir/browse.php?a_code=A-10-500-2&slc_lang=en&sid=1 Tahereh Jasemizad 10031947532846004873 10031947532846004873 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mehdi Mokhtari 10031947532846004874 10031947532846004874 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hengameh Zandi 10031947532846004875 10031947532846004875 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran Taher Shahriari 10031947532846004876 10031947532846004876 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran Fatemeh Sahlabadi fatemehsahlabadi@yahoo.com 10031947532846004877 10031947532846004877 Yes Shahid Sadoughi University of Medical Sciences, Yazd, Iran Akram Montazeri 10031947532846004878 10031947532846004878 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran Arefeh Dehghani Tafti 10031947532846004879 10031947532846004879 No Shahid Sadoughi University of Medical Sciences, Yazd, Iran