fa بهداشت Health پژوهشي Original Article <div style="text-align: justify;"><strong><em>Background and Purpose</em></strong>: The root extracts of<em> Dennettia tripetala</em> G. Baker and <em>Milicia excelsa</em> <a href="http://www.worldagroforestry.org/treedb/AFTPDFS/Milicia_excelsa.PDF" target="_blank">(Welw.) C. Berg</a> was investigated for their <em>in-vitro</em> antioxidant activities.<br> <strong><em>Materials and Methods</em></strong>: &nbsp;The pulverized roots of <em>Dennettia tripetala</em> and <em>Milicia excelsa</em> were extracted separately by cold maceration using ethyl acetate, methanol,<em> n</em>-butanol and water respectively as the extracting solvents. <em>In-vitro</em> antioxidant, the activity of root extracts was then investigated using DPPH model. The results obtained was analyzed using One-way Analysis of Variance (ANOVA) involving GraphPad Prism 5 Software.<br> <strong><em>Results</em></strong><strong>:</strong> The antioxidant assay of various extracts, using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging model, revealed that the ethanol and <em>n</em>-butanol extracts of <em>D. tripetala</em> had better radical scavenging activity with IC₅₀ values of 2.02 and 0.631 <em>&micro;</em>g/mL for ethanol and <em>n</em>-butanol extracts, respectively.&nbsp; The methanol and ethanol extracts of <em>M. excelsa</em> showed higher radical scavenging capacity with IC₅₀ of 0.194 and 8.84 <em>&micro;</em>g/mL compared to that of the ascorbic acid which had IC₅₀ value of 4.60 <em>&micro;</em>g/mL.<br> <strong><em>Conclusion</em></strong><strong>:</strong> The radical scavenging ability of the extracts could be attributed to the presence of flavonoids and phenolics.<br> &nbsp;</div> Phytochemicals, antioxidant, Dennettia tripetala, Milicia excelsa, DPPH 1 7 http://jhs.mazums.ac.ir/browse.php?a_code=A-10-650-2&slc_lang=fa&sid=1 Nwachukwu Ekere nwachukwu.ekere@unn.edu.ng 10031947532846006045 10031947532846006045 No University of Nigeria, Nsukka, Enugu State, Nigeria Tochukwu Oparanozie tochukwu.okparanozie@unn.edu.ng 10031947532846006046 10031947532846006046 No University of Nigeria, Nsukka, Enugu State, Nigeria Matthias Agbo matthias.agbo@unn.edu.ng 10031947532846006047 10031947532846006047 Yes University of Nigeria, Nsukka, Enugu State, Nigeria