Volume 9, Issue 4 (Autumn 2021)
Iran J Health Sci 2021, 9(4): 46-57 |
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Department of Physical Education and Sport Science, Technical and Vocational University (TVU), Tehran. Iran. , hamidreza.zolfi@yahoo.com
Abstract: (1625 Views)
Background and purpose: The aim of this study was to determine the effect of acute aerobic exercise and 14-days grape seed extract supplementation on total antioxidant capacity, lipid peroxidation, and muscle cell damage biomarkers in untrained males.
Materials and Methods: In a randomized, double-blinded, and placebo-controlled study, twenty-two male students (age 19±1 years, weight 67.44±7 kg, BMI 22±2, V̇O2max 39±2 ml/kg-1/min-1) were randomly assigned to two groups of grape seed extract and placebo (PLA) (GSE: 200 mg/day for two weeks). After subsequent 14-days of supplementation, subjects did a single session of aerobic exercise (running) on the treadmill at 75% VO2max for 30 minutes. Blood samples were taken 3 times: prior to supplementation (baseline), 14-days after supplementation, and immediately after exercise (post-exercise). Total antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH), and lactate were measured. TAC was determined by ABTs method. The collected data were then analyzed by running analysis of variance (ANOVA) with repeated measure and Bonferroni post-hoc tests as appropriate using SPSS17 at p<0.05.
Results: Malondialdehyde, CK, LDH, Lactate were significantly increased after aerobic exercise (p<0.05). Short-term GSE supplementation significantly prevented MDA and CK cascade after exercise compared to PLA (p<0.05) but, it had no significant effect on basal parameters (p>0.05). The exercise had also no significant effect on total antioxidant capacity in any of the groups (p>0.05).
Conclusion: Aerobic exercise could increase blood oxidative stress biomarkers and GSE supplementation, due to the influential antioxidant effect; yet, it could attenuate exercise-induced oxidative stress in men.
Materials and Methods: In a randomized, double-blinded, and placebo-controlled study, twenty-two male students (age 19±1 years, weight 67.44±7 kg, BMI 22±2, V̇O2max 39±2 ml/kg-1/min-1) were randomly assigned to two groups of grape seed extract and placebo (PLA) (GSE: 200 mg/day for two weeks). After subsequent 14-days of supplementation, subjects did a single session of aerobic exercise (running) on the treadmill at 75% VO2max for 30 minutes. Blood samples were taken 3 times: prior to supplementation (baseline), 14-days after supplementation, and immediately after exercise (post-exercise). Total antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH), and lactate were measured. TAC was determined by ABTs method. The collected data were then analyzed by running analysis of variance (ANOVA) with repeated measure and Bonferroni post-hoc tests as appropriate using SPSS17 at p<0.05.
Results: Malondialdehyde, CK, LDH, Lactate were significantly increased after aerobic exercise (p<0.05). Short-term GSE supplementation significantly prevented MDA and CK cascade after exercise compared to PLA (p<0.05) but, it had no significant effect on basal parameters (p>0.05). The exercise had also no significant effect on total antioxidant capacity in any of the groups (p>0.05).
Conclusion: Aerobic exercise could increase blood oxidative stress biomarkers and GSE supplementation, due to the influential antioxidant effect; yet, it could attenuate exercise-induced oxidative stress in men.
Keywords: Aerobic Exercise, Grape Seed Extract, Oxidative Stress, Antioxidant Effect, Supplementation
Type of Study: Original Article |
Subject:
Health
References
1. Taghizadeh M, Malekian E, Memarzadeh MR, Mohammadi AA, Asemi Z. Grape seed extract supplementation and the effects on the biomarkers of oxidative stress and Mmetabolic profiles in female volleyball players: A randomized, double-blind, placebo-controlled clinical trial. Iranian Red Crescent Medical Journal. 2016;18(9):e31314. [DOI:10.5812/ircmj.31314]
2. Yimcharoen M, Kittikunnathum S, Suknikorn C, Wichuda N, Petcharee Y, G. T, et al. Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise. Journal of the International Society of Sports Nutrition. 2019;16(2):1-9. [DOI:10.1186/s12970-019-0269-8] [PMID] [PMCID]
3. Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, et al. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology. 2000;148(2-3):187-97. [DOI:10.1016/S0300-483X(00)00210-9]
4. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clinical interventions in aging. 2018;13:757-72. [DOI:10.2147/CIA.S158513] [PMID] [PMCID]
5. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine. 2018;54(4):287-93. [DOI:10.1016/j.ajme.2017.09.001]
6. Bloomer RJ, Falvo MJ, Fry AC, Schilling BK, Smith WA, Moore CA. Oxidative stress response in trained men following repeated squats or sprints. Medicine & Science in Sports & Exercise. 2006;38(8):1436-42. [DOI:10.1249/01.mss.0000227408.91474.77] [PMID]
7. Park S-Y, Kwak Y-S. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of Exercise Rehabilitation. 2016;12(2):113-7. [DOI:10.12965/jer.1632598.299] [PMID] [PMCID]
8. Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or foe? Journal of sport and health science. 2020;9(5):415-25. [DOI:10.1016/j.jshs.2020.04.001] [PMID] [PMCID]
9. Jackson MJ, Pye D, Palomero J. The production of reactive oxygen and nitrogen species by skeletal muscle. Journal of Applied Physiology. 2007;102(4):1664-70. [DOI:10.1152/japplphysiol.01102.2006] [PMID]
10. Tucker WJ, Rosenberry R, Trojacek D, Chamseddine HH, Arena-Marshall CA, Zhu Y, et al. Studies into the determinants of skeletal muscle oxygen consumption: novel insight from near-infrared diffuse correlation spectroscopy. The Journal of Physiology. 2019;597(11):2887-901. [DOI:10.1113/JP277580] [PMID] [PMCID]
11. Finaud J, Lac G, Filaire E. Oxidative Stress. Sports Medicine. 2006;36(4):327-58. [DOI:10.2165/00007256-200636040-00004] [PMID]
12. Cooper C, Vollaard N, Choueiri T, Wilson M. Exercise, free radicals and oxidative stress. Biochemical Society Transactions. 2002;30(2):280-5. [DOI:10.1042/bst0300280] [PMID]
13. Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 2003;189(1-2):41-54. [DOI:10.1016/S0300-483X(03)00151-3]
14. Diaz KM, Feairheller DL, Sturgeon KM, Williamson ST, Brown MD. Oxidative stress response to short duration bout of submaximal aerobic exercise in healthy young adults. International Journal of Exercise Science. 2011;4(4):247-256.
15. Gawron-Skarbek A, Chrzczanowicz J, Kostka J, Nowak D, Drygas W, Jegier A, et al. Physical Activity, Aerobic Capacity, and Total Antioxidant Capacity in Healthy Men and in Men with Coronary Heart Disease. Oxidative Medicine and Cellular Longevity. 2015;2015:197307. [DOI:10.1155/2015/197307] [PMID] [PMCID]
16. Fisher-Wellman K, Bloomer RJ. Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine. 2009;8:1-25. [DOI:10.1186/1476-5918-8-1] [PMID] [PMCID]
17. Goldfarb AH, McKenzie MJ, Bloomer RJ. Gender comparisons of exercise-induced oxidative stress: influence of antioxidant supplementation. Applied Physiology Nutrition and Metabolism. 2007;32(6):1124-31. [DOI:10.1139/H07-078] [PMID]
18. Nazmi S, Fatma U, Zuhal H, Bekir Ç. Effects of acute twelve minute run test on oxidative stress and antioxidant enzyme activities. African Journal of Pharmacy and Pharmacology. 2011;5(9):1218-1222. [DOI:10.5897/AJPP11.263]
19. Ammar A, Chtourou H, Hammouda O, Turki M, Ayedi F, Kallel C, et al. Relationship between biomarkers of muscle damage and redox status in response to a weightlifting training session: effect of time-of-day. Physiology international. 2016;103(2):243-61. [DOI:10.1556/036.103.2016.2.11] [PMID]
20. Withee ED, Tippens KM, Dehen R, Tibbitts D, Hanes D, Zwickey H. Effects of Methylsulfonylmethane (MSM) on exercise-induced oxidative stress, muscle damage, and pain following a half-marathon: a double-blind, randomized, placebo-controlled trial. Journal of the International Society of Sports Nutrition. 2017;14(24):1-11. [DOI:10.1186/s12970-017-0181-z] [PMID] [PMCID]
21. Seifi-Skishahr F, Siahkohian M, Nakhostin-Roohi B. Influence of aerobic exercise at high and moderate intensities on lipid peroxidation in untrained men. The Journal of sports medicine and physical fitness. 2008;48(4):515-21.
22. Nakhostin-Roohi B, Babaei P, Rahmani-Nia F, Bohlooli S. Effect of vitamin C supplementation on lipid peroxidation, muscle damage and inflammation after 30-min exercise at 75% VO2max. The Journal of sports medicine and physical fitness. 2008;48(2):217-24.
23. Babaei P, Rahmani-Nia F, Nakhostin-Roohi B, Bohlooli S. The effect of VC on immunoendocrine and oxidative stress responses to exercise. Journal of Clinical and Diagnostic Research. 2009;3:1627-32.
24. Sacheck JM, Milbury PE, Cannon JG, Roubenoff R, Blumberg JB. Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free radical biology & medicine. 2003;34(12):1575-88. [DOI:10.1016/S0891-5849(03)00187-4]
25. Gonzalez-Izal M, Lusa Cadore E, Izquierdo M. Muscle conduction velocity, surface electromyography variables, and echo intensity during concentric and eccentric fatigue. Muscle & nerve. 2014;49(3):389-97. [DOI:10.1002/mus.23926] [PMID]
26. Thirumalai T, Therasa SV, Elumalai EK, David E. Intense and exhaustive exercise induce oxidative stress in skeletal muscle. Asian Pacific Journal of Tropical Disease. 2011;1(1):63-6. [DOI:10.1016/S2222-1808(11)60016-9]
27. Khani M, Motamedi P, Dehkhoda MR, Dabagh Nikukheslat S, Karimi P. Effect of thyme extract supplementation on lipid peroxidation, antioxidant capacity, PGC-1α content and endurance exercise performance in rats. Journal of the International Society of Sports Nutrition. 2017;14(11):1-8. [DOI:10.1186/s12970-017-0167-x] [PMID] [PMCID]
28. Pourghassem Gargari B, Abedini S, Babaei H, Aliasgarzadeh A, Pourabdollahi P. Effect of supplementation with grape seed (Vitis vinifera) extract on antioxidant status and lipid peroxidation in patient with type ΙΙ diabetes. Journal of Medicinal Plants Research. 2011;5(10): 2029-2034.
29. El-Ashmawy IM, Saleh A, Salama OM. Effects of marjoram volatile oil and grape seed extract on ethanol toxicity in male rats. Basic & clinical pharmacology & toxicology. 2007;101(5):320-327. [DOI:10.1111/j.1742-7835.2007.00125.x] [PMID]
30. Shi J, Yu J, Pohorly JE, Kakuda Y. Polyphenolics in grape seeds-biochemistry and functionality. Journal of medicinal food. 2003;6(4):291-299. [DOI:10.1089/109662003772519831] [PMID]
31. Grases F, Prieto RM, Fernández-Cabot RA, Costa-Bauzá A, Sánchez AM, Prodanov M. Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers. Nutrition Journal. 2015;14(1):94. [DOI:10.1186/s12937-015-0083-3] [PMID] [PMCID]
32. Sakr HF, Abbas AM, Bin-Jaliah I. Modulation of the neurological and vascular complications by grape seed extract in a rat model of spinal cord ischemia-Reperfusion injury by downregulation of both osteopontin and cyclooxygenase-2. Canadian Journal of Physiology and Pharmacology. 2016;94(7):719-27. [DOI:10.1139/cjpp-2015-0498] [PMID]
33. Belviranlı M, Gökbel H, Okudan N, Başaralı K. Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats. The British journal of nutrition. 2012;108(2):249-56. [DOI:10.1017/S0007114511005496] [PMID]
34. Najafi M, Vaez H, Zahednezhad F, Samadzadeh M, Babaei H. Study the effects of hydroalcoholic extract of grape seed (Vitis vinifera) on infarct size and cardiac arrhythmias in ischemic-reperfused isolated rat heart. Journal of Pharmaceutical Sciences. 2011;16(4):187-194.
35. Razmaraii N, Babaei H, Mohajjel Nayebi A, Assadnassab G, Ashrafi Helan J, Azarmi Y. Cardioprotective Effect of Grape Seed Extract on Chronic Doxorubicin-Induced Cardiac Toxicity in Wistar Rats. Advanced Pharmaceutical Bulletin. 2016;6(3):423-33. [DOI:10.15171/apb.2016.055] [PMID] [PMCID]
36. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical science. 1993;84(4):407-12. [DOI:10.1042/cs0840407] [PMID]
37. Carlsohn A, Rohn S, Mayer F, Schweigert FJ. Physical activity, antioxidant status, and protein modification in adolescent athletes. Medicine & Science in Sports & Exercise. 2010;42(6):1131-9. [DOI:10.1249/MSS.0b013e3181c74f7b] [PMID]
38. Carvalho J, Marques E, Ascensão A, Magalhães J, Marques F, Mota J. Multicomponent exercise program improves blood lipid profile and antioxidant capacity in older women. Archives of gerontology and geriatrics. 2010;51(1):1-5. [DOI:10.1016/j.archger.2009.05.020] [PMID]
39. Leelarungrayub D, Khansuwan R, Pothongsunun P, Klaphajone J. N-acetylcysteine supplementation controls total antioxidant capacity, creatine kinase, lactate, and tumor necrotic factor-alpha against oxidative stress induced by graded exercise in sedentary men. Oxidative Medicine and Cellular Longevity. 2011;329643. [DOI:10.1155/2011/329643] [PMID] [PMCID]
40. Kar P, Laight D, Rooprai HK, Shaw KM, Cummings M. Effects of grape seed extract in Type 2 diabetic subjects at high cardiovascular risk: a double blind randomized placebo controlled trial examining metabolic markers, vascular tone, inflammation, oxidative stress and insulin sensitivity. Diabetic medicine : a journal of the British Diabetic Association. 2009;26(5):526-31. [DOI:10.1111/j.1464-5491.2009.02727.x] [PMID]
41. Natella F, Belelli F, Gentili V, Ursini F, Scaccini C. Grape seed proanthocyanidins prevent plasma postprandial oxidative stress in humans. Journal of agricultural and food chemistry. 2002;50(26):7720-5. [DOI:10.1021/jf020346o] [PMID]
42. Moflehi D, Kok L-Y, Tengku-Kamalden T-F, Amri S. Effect of single-session aerobic exercise with varying intensities on lipid peroxidation and muscle-damage markers in sedentary males. Global Journal of Health Science. 2012;4(4):48-54. [DOI:10.5539/gjhs.v4n4p48] [PMID] [PMCID]
43. Bloomer RJ, Goldfarb AH, McKenzie MJ. Oxidative stress response to aerobic exercise: comparison of antioxidant supplements. Medicine & Science in Sports & Exercise. 2006;38(6):1098-105. [DOI:10.1249/01.mss.0000222839.51144.3e] [PMID]
44. Sano A, Uchida R, Saito M, Shioya N, Komori Y, Tho Y, et al. Beneficial effects of grape seed extract on malondialdehyde-modified LDL. Journal of nutritional science and vitaminology. 2007;53(2):174-82. [DOI:10.3177/jnsv.53.174] [PMID]
45. Shan Y, Ye X-H, Xin H. Effect of the grape seed proanthocyanidin extract on the free radical and energy metabolism indicators during the movement. Scientific Research and Essay. 2010; 5(2):148-153.
46. Saada HN, Said UZ, Meky NH, Abd El Azime AS. Grape seed extract Vitis vinifera protects against radiation-induced oxidative damage and metabolic disorders in rats. Phytotherapy research: PTR. 2009; 23(3):434-8. [DOI:10.1002/ptr.2684] [PMID]
47. Gleeson M. Biochemical and immunological markers of over-training. Journal of sports science & medicine. 2002;1(2):31-41.
48. Diaz E, Ruiz F, Hoyos I, Zubero J, Gravina L, Gil J, et al. Cell damage, antioxidant status, and cortisol levels related to nutrition in ski mountaineering during a two-day race. Journal of sports science & medicine. 2010;9(2):338-46.
49. Bulduk E, Ergene N, Baltacı AK, Gümüş H. Plasma antioxidant responses and oxidative stress following a 20 meter shuttle run test in female volleyball players. Journal of Human Sciences. 2011;8(2):510-27.
50. Marin DP, dos Santos Rde C, Bolin AP, Guerra BA, Hatanaka E, Otton R. Cytokines and oxidative stress status following a handball game in elite male players. Oxidative Medicine and Cellular Longevity. 2011;2011:804873. [DOI:10.1155/2011/804873] [PMID] [PMCID]
51. Büttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells. Journal of applied physiology. 2007;102(1):26-36. [DOI:10.1152/japplphysiol.00066.2006] [PMID]
52. Coco M, Perciavalle V. Creatine ingestion effects on oxidative stress in a steady-state test at 75% VO(2max). The Journal of sports medicine and physical fitness. 2012;52(2):165-9.
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