Volume 6, Issue 3 (Summer 2018)
Iran J Health Sci 2018, 6(3): 1-7 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ekere N, Oparanozie T, Agbo M. Effects of Solvents on the In-vitro Antioxidant Activity of Dennettia tripetala G. Baker and Milicia excelsa (Welw.) C. Berg Root Extracts. Iran J Health Sci 2018; 6 (3) :1-7
URL: http://jhs.mazums.ac.ir/article-1-578-en.html
URL: http://jhs.mazums.ac.ir/article-1-578-en.html
University of Nigeria, Nsukka, Enugu State, Nigeria , matthias.agbo@unn.edu.ng
Abstract: (2855 Views)
Background and Purpose: The root extracts of Dennettia tripetala G. Baker and Milicia excelsa (Welw.) C. Berg was investigated for their in-vitro antioxidant activities.
Materials and Methods: The pulverized roots of Dennettia tripetala and Milicia excelsa were extracted separately by cold maceration using ethyl acetate, methanol, n-butanol and water respectively as the extracting solvents. In-vitro antioxidant, the activity of root extracts was then investigated using DPPH model. The results obtained was analyzed using One-way Analysis of Variance (ANOVA) involving GraphPad Prism 5 Software.
Results: The antioxidant assay of various extracts, using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging model, revealed that the ethanol and n-butanol extracts of D. tripetala had better radical scavenging activity with IC50 values of 2.02 and 0.631 µg/mL for ethanol and n-butanol extracts, respectively. The methanol and ethanol extracts of M. excelsa showed higher radical scavenging capacity with IC50 of 0.194 and 8.84 µg/mL compared to that of the ascorbic acid which had IC50 value of 4.60 µg/mL.
Conclusion: The radical scavenging ability of the extracts could be attributed to the presence of flavonoids and phenolics.
Materials and Methods: The pulverized roots of Dennettia tripetala and Milicia excelsa were extracted separately by cold maceration using ethyl acetate, methanol, n-butanol and water respectively as the extracting solvents. In-vitro antioxidant, the activity of root extracts was then investigated using DPPH model. The results obtained was analyzed using One-way Analysis of Variance (ANOVA) involving GraphPad Prism 5 Software.
Results: The antioxidant assay of various extracts, using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging model, revealed that the ethanol and n-butanol extracts of D. tripetala had better radical scavenging activity with IC50 values of 2.02 and 0.631 µg/mL for ethanol and n-butanol extracts, respectively. The methanol and ethanol extracts of M. excelsa showed higher radical scavenging capacity with IC50 of 0.194 and 8.84 µg/mL compared to that of the ascorbic acid which had IC50 value of 4.60 µg/mL.
Conclusion: The radical scavenging ability of the extracts could be attributed to the presence of flavonoids and phenolics.
Type of Study: Original Article |
Subject:
Health
References
1. Doughari JH, Human IS, Bennade S, Ndakidemi DA. Phytochemicals as chemotherapeutic agents and antioxidants: Possible solution to the control of antibiotic resistant verocytotoxin-producing bacteria. Journal of Medicinal Plants Research 2009; 3(11):839-48.
2. Sultana B, Anwar F, Ashraf M. Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts. Molecules 2009; 14:2167-80. [DOI:10.3390/molecules14062167]
3. Khatoon M, Islam E, Islam R, Rahman AA, Khurshid Alam AHM, Khondkar P, Rashid M, Parvin S. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes 2013; 6:121. [DOI:10.1186/1756-0500-6-121]
4. Papas AM. Antioxidant Status, Diet, Nutrition and Health (pp 21-26). Boca Raton, FL: CRC Press, 1999.
5. Oyemitan IA. Evaluation of Dennettia tripetala G. Baker (Annonaceae) for Central Nervous System Activities. An M. Phil Thesis, Department of Pharmacology, Obafemi Awolowo University, Ile-Ife, Nigeria, 2006.
6. Burkill IH. The useful plants of West Tropical Africa. Families A-D (p 691). Royal Botanical Garden, Kew, 1985.
7. Udegbunam SO, Nnaji TO, Udegbunam RI, Okafor JC, Agbo I. Evaluation of herbal ointment formulation of Milicia excelsa for wound healing. Africa Journal of Biotechnology 2013; 12(21): 3351-59.
8. Iseghohi SO. A Review of the Uses and Medicinal Properties of Dennettia tripetala (Pepperfruit). Medical Sciences 2015; 3(4):104-11. [DOI:10.3390/medsci3040104]
9. Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry 2000; 55(6):481–504. [DOI:10.1016/S0031-9422(00)00235-1]
10. Agbo MO, Uzor PF, Akazie-Nneji UN, Eze-Odurukwe CU, Ogbatue, Mbaoji EC. Total Phenolic and Flavonoid contents in Selected Nigerian Medicinal Plants. Dhaka University Journal of Pharmaceutical Sciences 2015; 14(1): 35-41. [DOI:10.3329/dujps.v14i1.23733]
11. Yang, J, Chen, C, Zhao, S, Ge, F, Liu, D. Effect of Solvents on the Antioxidant Activity of Walnut (Juglans regia L.) Shell Extracts. Journal of Food and Nutrition Research 2014; 2(9): 621-26. [DOI:10.12691/jfnr-2-9-15]
12. Al-Farsi, M, Lee, MCY. Optimization of phenolic and dietary fibre extraction from date seeds. Food Chemistry 2008; 108(3):977–85. [DOI:10.1016/j.foodchem.2007.12.009]
13. Belmekki N, Bendimerad N. Antioxidant activity and phenolic content in methanol crude extracts from three Lamiaceae grown in southwestern Algeria. Journal of Natural Product and Plant Resources 2(1):175-81.
14. Mohd AA, Nurul HS, Almajano PM, Gallego GM. Solvent Effect on Antioxidant Activity and Total Phenolic Content of Betula alba and Convolvulus arvensis. International Journal of Biological, Bimolecular, Agricultural, Food and Biotechnological Engineering 2013; 7(5):152-57.
15. Okolie NP, Falodun A, Davids O. Evaluation of the Antioxidant Activity of Root Extract of Pepper Fruit (Dennetia Tripetala), and It's Potential for the Inhibition of Lipid Peroxidation. African Journal of Traditional, Complementary and Alternative Medicines 2014;11(3):221–27. [DOI:10.4314/ajtcam.v11i3.31]
16. Adedayo BC, Oboh G, Akindahunsi AA. Changes in the total phenol content and antioxidant properties of pepper fruit (Dennettia tripetala) with ripening. African Journal of Food Science 2010;4(6):403-09.
17. Van der Sluis, A.A, Dekker, M, Boekel, MAJS. Activity and concentration of polyphenolic antioxidants in apple juice stability during storage. Journal of Agricultural and Food Chemistry 2005; 53(4): 1073-80. [DOI:10.1021/jf040270r]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
