Volume 7, Issue 1 (Winter 2019)                   Iran J Health Sci 2019, 7(1): 9-17 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ezzati Ghadi F, Ramzani Ghara A, Rodbari Z. Ameliorating Effect Of Gamma Irradiated Chicory Against Carbon Tetra-Chloride Induced Kidney And Testis Damage In Rat. Iran J Health Sci. 2019; 7 (1) :9-17
URL: http://jhs.mazums.ac.ir/article-1-609-en.html
University of Jiroft, Jiroft, Iran , a.ramzani@ujiroft.ac.ir
Abstract:   (99 Views)
Background and purpose: Gamma radiation can affect the content of phytochemicals. The aim of this study was to investigate the ability of gamma irradiated chichorium intybus L. root extract to protect against carbon tetra-chloride (CCl4) induced kidneys and testes injury.
Materials and Methods: The rats were divided into six groups according to treatment: I) control, II) CCl4 (1ml/kg body weight by intraperitoneal injection (IP)), III) gamma irradiated chicory root extract (500 mg/kg body weight) + CCl4, IV) non-irradiated chicory root extract (500 mg/kg body weight) + CCl4, V) gamma irradiated chicory root extract, and VI) non-irradiated chicory root extract. The level of BUN and creatinine, and also histological study of kidney and testis tissues were estimated twenty-four hours after the last treatment at the end of four weeks.
Results: Gamma irradiated chicory root extract significantly decreased elevated level of BUN and creatinine in the serum of CCl4 treated rats. Histological evaluation revealed that gamma and non-irradiated chicory root extract treatment to CCl4 rats demonstrated reduce Bowman’s space and basement thickening of kidney. Moreover, the results showed the normalization of testes of CCl4 treated animals in group III and IV.
Conclusion: The gamma irradiated chicory root showed kidney and testis protection against CCl4 in rats. Based on the results of the present study, it can be concluded that chicory has a potent protective effect more than non-irradiated chicory root extract due to containing phenolic content.
Full-Text [PDF 678 kb]   (24 Downloads)    
Type of Study: Original Article | Subject: Health

1. Abdel Moneim AE. Prevention of carbon tetrachloride (CCl4)-induced toxicity in testes of rats treated with Physalis peruviana L. fruit. Toxicology and industrial health. 2016;32(6):1064–73. [DOI:10.1177/0748233714545502]
2. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reproductive Biology and Endocrinology. 2015;13(1):37. [DOI:10.1186/s12958-015-0032-1]
3. Agarwal A, Prabakaran SA. Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology. Indian Journal of Experimental Biology. 2005 Nov;43(11):963-74. [PMID: 16315393].
4. Wathes DC, Abayasekara DRE, Aitken RJ. Polyunsaturated fatty acids in male and female reproduction. Biology of Reproduction. 2007;77(2):190–201. [DOI:10.1095/biolreprod.107.060558]
5. Abraham P, Wilfred G. Oxidative damage to the lipids and proteins pf the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clinica chimica acta; international journal of clinical chemistry. 1999;289(1–2):177–9. [PMID: 10636716].
6. Doğukan A, Akpolat N, Çeliker H, Ilhan N, Halil IB, Günal AI. Protective effect of interferon-alpha on carbon tetrachloride-induced nephrotoxicity. Journal of Nephrology. 2003;16(1):81–4. [PMID: 12649538].
7. Saeed M, Baloch AR, Wang M, Soomro RN, Baloch AM, Bux BA, et al. Use of Cichorium Intybus Leaf extract as growth promoter, hepatoprotectant and immune modulent in broilers. Journal of Animal Production Advances. 2015;5(1):585–91. [DOI:10.5455/japa.20150118041009]
8. Shad MA, Nawaz H, Rehman T, Ikram N. Determination of some biochemicals, phytochemicals and antioxidant properties of different parts of Cichorium intybus L.: A comparative study. Journal of Animal Plant Scince. 2013;23(4):1060–6. ISSN: 1018-7081.
9. Antonio AL, Carocho M, Bento A, Quintana B, Botelho ML, Ferreira ICFR. Effects of gamma radiation on the biological, physico-chemical, nutritional and antioxidant parameters of chestnuts–A review. Food Chemistry Toxicology. 2012;50(9):3234–42. [DOI:10.1016/j.fct.2012.06.024]
10. Diehl JF. Food irradiation—past, present and future. Radiation Physics and Chemistry. 2002;63(3–6):211–5. [DOI:10.1016/S0969-806X(01)00622-3]
11. Harrison K, Were LM. Effect of gamma irradiation on total phenolic content yield and antioxidant capacity of almond skin extracts. Food Chemistry. 2007;102(3): 932–7. [DOI:10.1016/j.foodchem.2006.06.034]
12. Li G-Y, Gao H-Y, Huang J, Lu J, Gu J-K, Wang J-H. Hepatoprotective effect of Cichorium intybus L., a traditional Uighur medicine, against carbon tetrachloride-induced hepatic fibrosis in rats. World Journal of Gastroenterology. 2014; 20(16):4753. [DOI:10.3748/wjg.v20.i16.4753]
13. Heibatollah S, Reza NM, Izadpanah G, Sohailla S. Hepatoprotective effect of Cichorium intybus on CCl4-induced liver damage in rats. African Journal of Biochemistry Research. 2008;2(6):141–4.
14. Mossa A-TH, Swelam ES, Mohafrash SMM. Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histopathological changes in the liver and kidney of male albino rats. Toxicology reports. 2015;2:775–84. [DOI:10.1016/j.toxrep.2015.02.009]
15. Humanson GL. Basic procedures—animal tissue technique. Animal tissue techniques. WH Freeman & Co., San Francisco; 1961.
16. Mukai T, Mera K, Nishida K, Nakashima M, Sasaki H, Sakaeda T, et al. A novel method for preparation of animal models of liver damage: liver targeting of carbon tetrachloride in rats. Biological and Pharmaceutical Bulletin. 2002;25(11): 1494–7. [PMID: 12419969]. [DOI:10.1248/bpb.25.1494]
17. Sanzgiri UY, Srivatsan V, Muralidhara S, Dallas CE, Bruckner J V. Uptake, distribution, and elimination of carbon tetrachloride in rat tissues following inhalation and ingestion exposures. Toxicology and applied pharmacology. 1997;143(1):120–9. [DOI:10.1006/taap.1996.8079]
18. Orhue NE, Nwanze EA. Increases in plasma urea and creatinine in experimental Trypanosoma brucei infection is influenced by Scoparia dulcis. Annals of Biomedical Sciences. 2005;4(1):35–41.
19. Adaikpoh MA, Orhue NE. Cadmium-induced hepatorenal toxicity in rats: Possible ameliorative effects of Talinum triangulare. NISEB J. 2012;12:21–6.
20. Garcia-Cohen E-C, Marin J, Diez-Picazo LD, Baena AB, Salaices M, Rodriguez-Martinez MA. Oxidative stress induced by tert-butyl hydroperoxide causes vasoconstriction in the aorta from hypertensive and aged rats: role of cyclooxygenase-2 isoform. Journal of Pharmacology and Experimental Therapeutics. 2000;293(1):75–81. PMID: 10734155.
21. Sahreen S, Khan MR, Khan RA, Alkreathy HM. Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat. Food Nutrition Research. 2015;59(1): 28438. [DOI:10.3402/fnr.v59.28438]
22. Tirkey N, Pilkhwal S, Kuhad A, Chopra K. Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC Pharmacology. 2005;5(1):2. https://doi.org/10.1186/1471-2210-5-15 [DOI:10.1186/1471-2210-5-2]
23. Kalantari, H., Danesh Pajou, M., Kheradmand, P., Goodarzian, M., & Zeidooni, L. (2018). Nephroprotective Effect of Hydroalcoholic Extract Allium jesdianum Boiss against Carbon Tetrachloride Induced Nephrotoxicity via Stress Oxidative in Mice: Neproprotective effect of Allium against CCl4. Pharmacology, 2018; 24(2).
24. Hismiogullari AA, Hismiogullari SE, Karaca O, Sunay FB, Paksoy S, Can M, et al. The protective effect of curcumin administration on carbon tetrachloride (CCl4)-induced nephrotoxicity in rats. Pharmacology Reports. 2015;67(3):410–6. [DOI:10.1016/j.pharep.2014.10.021]
25. Ustyol L, Demirören K, Kandemir I, Erten R, Bulan K, Kaba S, et al. Comparative nephroprotective effects of silymarin, N-acetylcysteine, and thymoquinone against carbon tetrachloride-induced nephrotoxicity in rats. Iranian Red Crescent Medical Journal.2017;19(1). [DOI:10.5812/ircmj.37746]
26. Khan RA, Khan MR, Sahreen S. Protective effects of rutin against potassium bromate induced nephrotoxicity in rats. BMC complementary and alternative medicine. 2012;12(1):204. [DOI:10.1186/1472-6882-12-204]
27. Queiroz M-JRP, Ferreira ICFR, Calhelha RC, Estevinho LM. Synthesis and antioxidant activity evaluation of new 7-aryl or 7-heteroarylamino-2, 3-dimethylbenzo [b] thiophenes obtained by Buchwald–Hartwig C–N cross-coupling. Bioorganic & medicinal chemistry. 2007;15(4):1788–94. [DOI:10.1016/j.bmc.2006.11.035]
28. Hanafi N. CCI4 Model: Role of BM Transplantation. Journal of Biological Sciences. 2012;12(3):128–37.
29. Khan RA. Protective effects of Launaea procumbens on rat testis damage by CCl 4. Lipids in health and disease. 2012;11(1):103. [DOI:10.1186/1476-511X-11-103]
30. Foaud MA, Kamel AH, El-Monem DDA. The protective effect of N-acetyl cysteine against carbon tetrachloride toxicity in rats. The Journal of Basic and Applied Zoology. 2018;79(1):14. [DOI:10.1186/s41936-018-0022-x]
31. Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, et al. The protective ability of Mediterranean dietary plants against the oxidative damage: The role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol contents. Food Chemistry. 2009; 112(3):587–94. [DOI:10.1016/j.foodchem.2008.06.013]
32. Ezzati Ghadi F, Ramzani Ghara A, Esmaeilipour O, Kohannia N. Evaluation of Gamma Ray Effects on Total Phenolic Contents, Antioxidants and Antibacterial Activities of Cichorium Intybus L. Biomacromolecular Journal. 2017; 3(2), 115-122.
33. Vardhan PV, Shukla LI. Gamma irradiation of medicinally important plants and the enhancement of secondary metabolite production. International journal of radiation biology. 2017; 93(9), 967-979. [DOI:10.1080/09553002.2017.1344788]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2019 All Rights Reserved | Iranian Journal of Health Sciences

Designed & Developed by : Yektaweb